Localization of ASH1 mRNA particles in living yeast : ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3'UTR (untranslated region) sequences. Courtesy: Robert Singer Mgat1-knockout mouse at day 9 of development: This is a mutant mouse embryo that cannot make complex N-glycans. It is stained with rhodamine-ConA which detects oligomannosyl N-glycans that are increased in this mutant. The mutant embryo cannot develop beyond mid-gestation showing that complex or hybrid N-glycans are essential for mammalian development. Courtesy: Pamela Stanley Crystal structure of the homotrimeric globular head domain for Acrp30, a secretory protein that is predominantly released from adipocytes. This structure reveals an unexpected homology to the tumor necrosis factor family of cytokines, despite the lack of homology at the primary amino acid sequence level. Both proteins have a ten-strand jelly-roll folding topology and form bell-shaped homotrimeric oligomers. Dr. Scherer's group is studying the role of Acrp30 in fine tuning tissue response to insulin. Courtesy: Philipp Scherer

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