Michael-Christopher Keogh, Ph.D
Chanin Bldg., Room 415
(718) 430-8796
mkeogh@aecom.yu.edu
Research interestsWe will investigate the role of chromatin in RNA Polymerase II (RNApII) transcription elongation, DNA Double-Strand Break (DSB) repair and chromosome stability.
Recent publications* Joint First Authorship
(19) Fillingham J, Keogh M-C & Krogan NJ (2006) γH2AX and its role in DNA double-strand break repair. Biochem Cell Biol 84:559-68. <PDF>
(18) Keogh M-C, Mennella TA, Sawa C, Berthelet S, Krogan NJ, Wolek A, Podolny V, Carpenter LR, Greenblatt JF, Baetz K & Buratowski S (2006) The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4. Genes Dev 20:660-5. <PDF>
(17) Keogh M-C*, Kim J-A*, Downey M*, Fillingham J, Chowdhury D, Harrison JC, Onishi M, Datta N, Galicia S, Emili A, Lieberman J, Shen X, Buratowski S, Haber JE, Durocher D, Greenblatt JF & Krogan NJ (2006) A phosphatase complex that dephosphorylates γH2AX regulates DNA damage checkpoint recovery. Nature 439:497-501 (2005 doi:10.1038/nature04384)
The significance of this paper and related work on PP2A (#16) are discussed in a Nature News & Views (439:406-7) and Cell Molecular Biology Select article (124:227).
<PDF> <Supplementary> <Nature N&V> <Cell Molecular Biology Select>(16) Chowdhury D, Keogh M-C, Ishii H, Peterson CL, Buratowski S & Lieberman J (2005) γH2AX dephosporylation by Protein Phosphatase 2A facilitates DNA double-strand break repair. Mol Cell 20:801-9. <PDF> <Supplementary> <Nature N&V> <Cell Molecular Biology Select>
(15) Keogh M-C, Kurdistani S, Morris SA, Ahn SH, Podolny V, Collins SR, Shuldiner M, Chin K, Punna T, Thompson NJ, Boone C, Emili A, Weissman JS, Hughes TR, Strahl BD, Grunstein M, Greenblatt JF, Buratowski S & Krogan NJ (2005) Co-transcriptional Set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. Cell 123:593-605.
The significance of this paper is discussed in a Cell Minireview (123:1187-90). <PDF>
<Supplementary> <Cell MiniReview> <Harvard Focus>(14) Starita LM*, Horwitz AA*, Keogh M-C, Ishioka C, Parvin JD & Chiba N (2005) BRCA1 / BARD1 ubiquitinate phosphorylated RNA Polymerase II. J Biol Chem 280:24498-505. <PDF>
(13) Krogan NJ*, Lam MHY*, Fillingham J*, Keogh M-C*, Gebbia M, Li J, Datta N, Wu X, Cagney G, Buratowski S, Emili A & Greenblatt JF (2004) Proteasome involvement in the repair of DNA double-strand breaks. Mol Cell 16:1027-34. <PDF>
(12) Krogan NJ*, Baetz K*, Keogh M-C*, Datta N, Sawa C, Kwok TCY, Thompson NJ, Davey MG, Pootoolal J, Hughes TR, Emili A, Buratowski S, Hieter P & Greenblatt JF (2004) Regulation of chromosome stability by the Histone H2A variant Htz1, the Swr1 chromatin remodeling complex and the Histone AcetylTransferase NuA4. Proc Natl Acad Sci USA 101:15313-8. <PDF>
(11) Keogh M-C & Buratowski S (2004) Using chromatin immunoprecipitation to map co-transcriptional mRNA processing in Saccharomyces cerevisiae. Methods Mol Biol 257:1-16.
(10) Krogan NJ*, Keogh M-C*, Datta N, Sawa C, Ryan OW, Ding H, Haw RA, Pootoolal J, Tong A, Canadien V, Richards DP, Wu X, Emili A, Hughes TR, Buratowski S & Greenblatt JF (2003) A Snf2-family ATPase complex required for recruitment of the Histone H2A variant Htz1. Mol Cell 12:1565-76.
The significance of this paper is discussed in a Science Perspectives article (303:324-5) and a Cell Minireview (117:5-7). <PDF> <Science Perspectives> <Cell MiniReview>
(9) Keogh M-C, Podolny V & Buratowski S (2003) Bur1 kinase is required for efficient transcription elongation by RNA Polymerase II. Mol Cell Biol 23:7005-18.
(8) Takagi T, Cho E-J, Janoo RTK, Podolny V, Takase Y, Keogh M-C, Woo S-A, Fresno-Cohen LD, Hoffman CS & Buratowski S (2002) Divergent subunit interactions among fungal mRNA 5'-capping machineries. Euk Cell 3:448-57.
(7) Keogh M-C, Cho E-J, Podolny V & Buratowski S (2002) Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation. Mol Cell Biol 22:1288-97.
(6) Rodriguez CR, Cho E-J, Keogh M-C, Moore CL, Greenleaf AL & Buratowski S (2000) Kin28, the TFIIH-associated CTD kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol 20:104-12.
(5) Keogh M-C & Chen D (1999) Efficient liposome-mediated gene transfer to rabbit carotid arteries in vivo. In Methods in Molecular Medicine - Vascular Disease: Molecular Biology and Gene Therapy Protocols. Humana Press. Ed. AH Baker.
(4) Schmitt JF*, Keogh M-C*, Chen D, Taylor T, Weston K, Lupu F, Kakkar VV & Lemoine NR (1999) Tissue-selective dominant-negative proteins for the regulation of vascular smooth muscle cell proliferation. Gene Therapy 6:1184-91.
(3) Keogh M-C, Chen D, Schmitt JF, Dennehy U, Kakkar VV & Lemoine NR (1999) Design of a muscle-cell specific expression vector utilizing human vascular smooth muscle α-actin regulatory elements. Gene Therapy 6: 616-28.
(2) Keogh M-C*, Chen D*, Shaper N, Lupu F, Schmitt JF, Kakkar VV & Lemoine NR (1997) High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid-DNA complex. Gene Therapy 4: 162-71.
(1) Keogh M-C, Elliot J, Norton T & Lake RA (1997) A function for CD2 on murine B cells? Immunol Cell Biol 75: 333-9.
Michael Keogh: Research interests | BiosketchFaculty research at a glance
Birshtein | Bouhassira | Edelmann | Fyodorov | Keogh | Kielian | Kitsis | Nathenson | Query
Scharff | Schildkraut | Shafritz | Singer | Skoultchi | Stanley | Steidl |Warner | Ye
