The Tuberculosis Research Group at AECOM has the resources and equipment necessary for designing and carrying out sophisticated experiments and for implementing new and modern methods and protocols in a wide range of areas concerning the molecular genetics and cell biology of mycobacteria. The group is operating a Bio-Safety Level 3 (BSL3) Lab, with one of the very best aerosol chambers installed. These resources enable us to design different models of the tuberculosis infection and to study in vivo the causative agent Mycobacterium tuberculosis and its pathogenicity.

Molecular Genetics

Our experiments in molecular genetics are mainly designed and performed in Dr. Jacobs's Lab. The aim here is to create and implement the tools for the mycobacterial genetics, such as: construction of new plasmid and phasmid vectors, creating null mutants of M. tuberculosis and M. smegmatis in different metabolic pathways; construction of genomic libraries; transposon delivery systems; site-specific recombination systems; design of new vaccine candidate strains; and many others.
        - Molecular genetics protocols
        - Construction of M. tuberculosis knock-out library -- "4049 genes - 4049 knock-outs"
        - Ordered M. tuberculosis Genomic Library of Cosmids
        - Specialized transduction
        - Important Strains and Vectors
        - List of M. tuberculosis knockouts
        - List of complementing M. tuberculosis cosmids

Cell Biology

        - Cell - Biology Protocols

Bio-Safety Level 3 (BSL3) Lab

        - The Lab
        - The Aerosol Infection Chamber
        - BSL3 protocols

Core Equipment

        - Microscopy
        - FACS Analysis
        - HPLC
        - Aerosol Infection Chamber
        - Anaerobic Chamber
        - Computer Room

Protocols, Strains, Vectors and Cell-lines

        Protocols
                - molecular genetics protocols
                - cell-biology protocols
                - BSL3 protocols
        Strains
        Vectors
        Cell-lines

For more information about this site, please contact Dr. Jordan Kriakov
File Last Updated: Wednesday, March 14, 2007